HPMI
HIGH
PERFORMANCE
MOLECULAR
IMAGING
- Hong Zhou Viral Reconstructions of Herpes.
My research interest focuses on developing high performance image
processing software for the 3D structural determination of viruses
from electron cryomicroscopy (cryoEM) images. Structural determination
from cryoEM images consists three major steps: particle orientation and
center determination, orientation and center refinement, and 3D
reconstruction. My current project is to develop novel algorithms
for the orientation and center refinement of high resolution (but
noisy) cryoEM images.
- Amy McGough Helical Reconstructions of Actin.
My research involves structural studies of a protein called
gelsolin which binds to and breaks actin filaments as part of its
normal function in living cells. This ability to sever filaments makes
gelsolin a key player in cellular processes such as motility and
differentiation, both of which rely on dramatic reorganizations of the
actin cytoskeleton. In addition, gelsolin holds promise as a
therapeutic agent for cystic fibrosis by acting as a mucolytic agent
for CF sputum.
- Karen Haskell
- Ridgway Scott
Algorithms for enhanced resolution.
In collaboration with Professor Wah Chiu of the Baylor
College of Medicine, we are attempting to develop new algorithms
for improved imaging of electron micro-graphs. Electron microscopy
is an exciting technique for the discovery of the biological structure
of large proteins. Current methods limit the reconstruction to a
resolution several times greater than the physical resolution of the
electron microscope. We hope both to improve the resolution and to
speed the process of data reduction. Ultimately, we hope to change the
character of structural imaging so that large numbers of high-resolution
structures can be determined in the amount of time now required for a
single low-resolution image. Since knowledge of the basic structure of
macromolecular assemblies, such as viruses and cytoskeletal components,
leads to a deeper understanding of their function, this will open the
door to a host of new biomedical advances.
- Lamture, J.B., Zhou, Z.H., Kumar, A.S., Wensel, T.G. (1995)
Luminescence properties of Terbium (III) complexes with 4-substituted
dipicolinic acid analogues, Inorganic Chem.,
34, No. 4. 864-869.
- Lu, G.Y., Zhou, Z.H., Jakana, J., Cai, D.Y., Chen, S.X., Wei,
X.C., Gu, X.C., and Chiu, W. (1995) Three-dimensional Structure of Rice Dwarf
Virus by Electron Cryomicroscopy, High Tech. Let.
5, No 1,1-4.
- McGough, A. and M. Way. 1995. Molecular model of an actin filament capped by a
severing protein. J. Struct. Biol. 115:144-150.
- McGough, A., M. Way, and D. DeRosier. 1994. Determination of the alpha-actinin
binding site on actin filaments by cryoelectron microscopy and image analysis.
J. Cell Biol. 126:433-443.
- McGough, A. and R. Josephs. 1990. On the structure of erythrocyte spectrin in
partially expanded membrane skeletons. Proc. Natl. Acad. Sci.
87:5208-5212.
- Zhou, Z.H., Hardt, S., Wang, B., Sherman, M.B., Joanita, J., Chiu, W. (1996)
CTF determination of images of ice-embedded single particles using a graphics
interface, J. Struct. Biol. Vol. 115 in the press.
- Zhou, Z.H., He, J., Jakana, J., Tatman, J.D., Rixon, F., Chiu, W. (1995)
Assembly of VP26 in herpes simplex virus-1 inferred from structures of
wild-type and recombinant capsids, Nature Struct. Biol.
Vol. 2, No. 11. 1026-1030.
- Zou, Z.H., Prasad, B.V.V., Jakana, J., Rixon, F., Chiu, W. (1994) Protein
subunit structures in the herpes simplex virus A-capsid determined from 400 kV
spot-scan electron cryomicroscopy, J. Mol. Biol., 242:456-469.
- Zhou, Z.H., Chiu, W. (1993) Prospects for using an IVEM with a FEG for imaging
macromolecules towards atomic resolution, Ultramicroscopy 49:
407-416.
- Chiu, W., Jakana, J., Zhou, Z.H., Thuman-Commike, P. (1995) 400 keV
electron cryomicroscopy and computer reconstruction of spherical viruses, June
4-7, Ottawa, Canada.
- DeRosier, D., A. McGough, L. A. Melanson, D. Morgan, and C. Owen. 1994. High
resolution studies of helical structures by electron cryomicroscopy.
Proceedings of the 13th International Congress on Electron Microscopy.
Paris, FRANCE.
- Lu, G.Y., Zhou, Z.H., Jakana, J., Cai, D.Y., Chen, S.X., Gu, X.C., Chiu, W.
(1994) Three-dimensional structure of rice dwarf virus determined by
electron cryomicroscopy. The Third Asia-Pacific congress of medical virology,
p.p. 252, Oct. 23-28, Beijing, China.
- McGough, A. and R. Josephs. 1992. Electron microscopy and image reconstruction
reveal the structural basis for spectrin's elastic properties. Proceedings
of the Electron Microscopy Society of America. Boston, Massachusetts.
- McGough, A and R. Josephs. 1990. The helical structure of erythrocyte spectrin:
Implications for red cell elasticity. J. Cell. Biochem. Suppl.
14a: 240.
- McGough, A. and R. Josephs. 1989. The structure of partially expanded
erythrocyte spectrin. Proceedings of the Electron Microscopy Society of
America. San Antonio, Texas.
- McGough, A., B. Carragher, and R. Josephs. 1989. Image analysis reveals
periodic structure in negatively stained spectrin. Biophys. J.
55: 216a.
- Zhou, Z.H., He, J., Jakana, J., Tatman, J.D., Rixon, F.J., Chiu, W.
(1995) Assembly of VP26 in herpes simplex virus-1 capsid implicated by
3D structure of recombinant capsid, Proc. 53rd Annu. Meeting of the Micros.
Soc. of America. Aug. 13-17, Kansas City, MO.
- Zhou, Z.H., He, J., Jakana, J., Tatman, J.D., Rixon, F.J., Chiu, W. (1995)
Defining interaction and assembly of VP26 in herpes capsid from 3d structure of
recombinant capsids,20th International Herpesvirus Workshop, July 29 - Aug. 3,
Groninger, The Netherlands.
- Zhou, Z.H., He, J., Jakana, J., Tatman, J.D., Rixon, F.J., Chiu, W. (1995)
Domain structures of VP26 and VP5 determined by electron cryomicroscopy of
recombinant and naturally occurring HSV-1 capsids, The 14th annual meeting of
American Society for Virology, p.p. 200, July 8-12, Austin, TX.
- Zhou, Z.H., He, J., Jakana, J., Tatman, J.D., Rixon, F.J., Chiu, W. (1995)
Assembly of herpesvirus capsids inferred from the 3D structures
by 400keV spot-scan electron cryomicroscopy, Gordon Research Conference - Three
Dimensional Electron Microscopy of Macromolecules, June 25-30,
New Hampton School, NH
- Zhou, Z.H., Jakana, J., Prasad, B.V.V., Rixon, F.J., Chiu, W. (1995)
Structural variations between herpes simplex virus capsids at different
maturation stpes observed by electron cryomicroscopy,West Coast
Crystallography Workshop, March 12-15, Montarey, CA.
- Zhou, Z.H., Prasad, B.V.V., Jakana, J., Rixon, F.J., Chiu, W. (1994) Protein
subunit structure of herpes simplex virus A-capsid determined from 400-kV
electron cryomicroscopy. The Third Asia-Pacific congress of medical virology,
p.p. 239, Oct. 23-28, Beijing, China.
- Zhou, Z.H., Prasad, B.V.V., Jakana, J., Rixon, F.J., Chiu, W. (1994) Protein
subunit structure of herpes simplex virus A-capsid determined from 400-kV
electron cryomicroscopy. The 19th international herpesvirus workshop,
July 30 - Aug. 5, Vancouver, Canada.
- Zhou, Z.H., Prasad, B.V.V., Jakana, J., Rixon, F.J., Chiu, W. (1994) Protein
subunit structures in capsomeres of herpes simplex virus capsid determined by
400kV spot-scan electron cryomicroscopy. The 13th annual meeting of American
Society for Virology, p.p. 108, July 9-13, Madison, WI.
- Zhou, Z.H., Jakana, J., Prasad, B.V. V., Rixon, F.J., Chiu, W. (1994) Subunit
structures in capsomeres of herpes simplex virus capsid revealed by 400kV
spot-scan electron cryomicroscopy, Biophysical J., Vol. 66 No. 2,
pt. 2, A135. 38th Annual Meeting of the Biophysical Society, New Orleans, LA.
961119 JBV